Virulent bacteria

Nigella sativa L seeds oil concentration after overnight
incubation at 37oC

5%

10%

15%

20%

Staphylococcus aureus

0.9±0.1

0.8±0.2

0.7±0.1

0.6±0.2

Streptococcus pyogenes

0.9±0.1

0.8±0.2

0.7±0.1

0.6±0.2

Streptococcus pneumoniae

0.9±0.2

0.8±0.2

0.7±0.1

0.6±0.2

Listeria monocytogenes

0.9±0.1

0.8±0.2

0.7±0.1

0.6±0.2

Escherichia coli

0.9±0.1

0.9±0.1

0.8±0.2

0.7±0.1

Klebsiella pneumoniae

0.9±0.1

0.9±0.1

0.9±0.1

0.8±0.2

Salmonella typhi

0.9±0.1

0.9±0.1

0.9±0.1

0.8±0.2

Pseudomonas aeruginosa

0.9±0.2

0.9±0.2

0.9±0.1

0.8±0.2

Table 1: Turbidity signal of virulent bacteria after overnight incubation at 37oC

Virulent bacteria

Nigella sativa L seeds oil concentration after overnight
incubation at 37oC

5%

10%

15%

20%

Staphylococcus aureus

1.0±0.3

0.7±0.1

0.4±0.2

0.2±0.2

Streptococcus pyogenes

1.0±0.2

0.6±0.2

0.4±0.2

0.2±0.1

Streptococcus pneumoniae

1.0±0.1

0.7±0.2

0.5±0.2

0.2±0.3

Listeria monocytogenes

1.1±0.1

0.7±0.2

0.5±0.1

0.2±0.3

Escherichia coli

1.1±0.1

1.1±0.1

0.8±0.2

0.5±0.3

Klebsiella pneumoniae

1.6±0.2

1.6±0.2

1.6±0.1

1.3±0.2

Salmonella typhi

1.6±0.3

1.5±0.2

1.4±0.1

1.2±0.1

Pseudomonas aeruginosa

1.6±0.4

1.5±0.3

1.5±0.2

1.3±0.1

Table 2: Biomass wet weight by gram of virulent bacteria after overnight incubation at 37oC

Virulent bacteria

Nigella sativa L seeds oil concentration after overnight
incubation at 37oC

5%

10%

15%

20%

Staphylococcus aureus

0.6±0.1

0.4±0.2

0.2±0.2

0.1±0.2

Streptococcus pyogenes

0.6±0.2

0.4±0.1

0.3±0.1

0.1±0.1

Streptococcus pneumoniae

0.6±0.2

0.5±0.2

0.3±0.2

0.1±0.1

Listeria monocytogenes

0.7±0.1

0.5±0.1

0.3±0.1

0.1±0.1

Escherichia coli

0.7±0.2

0.7±0.1

0.5±0.1

0.3±0.1

Klebsiella pneumoniae

1.0±0.1

1.0±0.1

0.9±0.2

0.8±0.2

Salmonella typhi

0.9±0.2

0. 9±0.1

0.9±0.1

0.7±0.2

Pseudomonas aeruginosa

1.0±0.2

0.9±0.2

0.9±0.2

0.8±0.2

Table 3: Biomass dry weight by gram of virulent bacteria after overnight incubation at 37oC