164. Sheep heart, 165.Sheep heart, 166.Sheep heart, 167. Sheep liver, 168. Sheep heart, 169. Goat liver, 170. Sheep heart, 171. Goat heart, 172. Chicken brain, 173.Chicken brain, 174.Fish muscle, 175.Goat brain, 176.Chicken brain, 177.Fish muscle, 178.Sheep liver, 179. Fish muscle, P-Positive PCR with PTG strain of T.gondii, N-Negative PCR, M-Molecular Marker-100 bp gene ruler (Thermo Fisher Scientific i.e. ranges from 100 bp to 1500 bp).
Figure 1: Showing the gel image of the results of the direct detection of B1 nested PCR (499 bp) amplification of Toxoplasma gondii from different meat and offal such as liver, heart and brain collected from Karnataka

S.No

Species

Liver

Heart

Liver + heart

Brain

Muscle

Places of collection

1.

Sheep

4

3

9

07

-

Boty Bazaar, Mysore

7

4

14

-

-

Saraswathipuram, Mysore

22

20

20

-

-

Nazarbad, Mysore

2.

Goat

3

1

5

5

-

Boty Bazaar, Mysore

5

7

8

-

-

Nazarbad, Mysore

3.

Fishes

-

-

-

-

41

Boty Bazaar, Mysore

4.

Chicken

-

-

-

13

-

Gulbarga (Northern Karnataka)

 

 

-

-

-

20

-

Bidar (Northern Karnataka)

Table 1:Showing the details of sampling from different food animals from retail mutton and fish stalls from Mysore City (Southern Karnataka), Gulbarga and Bidar (Northern Karnataka)

Oligonucleotides

Sequence

position

Size of the PCR product

Outer forward primer

5'-ACA GAA AGG GAG CAA GAG TT-3'

12-31

 

Outer reverse primer

5'-CTG GGT TGG CTG AAA GAT A-3'

1478-1496

1484 bp

Internal forward primer

5'-AGG GTA CGT GTT GCA TCA T- 3'

453-471

 

Internal reverse primer

5'-TCT CCG CAG CGA CTT CTA T-3'

934-952

499 bp

Table 2:Details of B1 gene specific oligonucleotides

S.No.

Sheep

Goat

Fishes

Chicken

Total

Overall % prevalence

 

Liver

19 (33)

2 (08)

-

-

 

 

 

Heart

04 (27)

3 (08)

-

-

 

 

 

Liver and heart

08 (43)

6 (13)

-

-

 

 

 

Brain

01 (07)

2 (05)

-

2 (33)

 

 

 

Muscle

-

-

2 (41)

-

 

 

 

Total

32 (110⃰)

13 (34⃰ ⃰)

2 (41†)

2 (33††)

49 (218)

 

 

Species Wise Percent Prevalence

29.09%

38.23%

4.87%

6.06%

 

 

22.47%

* total numbers of genomic DNA from samples of sheep tissues

** total numbers of genomic DNA from samples of goat tissues

†total numbers of genomic DNA from samples of fishes

††total numbers of genomic DNA from samples of chicken

Table 3:Details of B1 gene specific oligonucleotides