A. Ocimum sanctum extract (OSE); B. silver nitrate (SN); C. Silver nanoparticles synthesised with Ocimum sanctum (AgNP);
Aa : Aggregatibacter actinomycetemcomitans
Figure 1: Shows the Minimum Bactericidal Concentrations of OSE, SN, AgNP against Aa at 100 μg/ml (left column) and 3.13μg/ml (right column).

Serial
dilutions (μg/ml)
Fn
Pg
Aa
Pi
OSE
SN
AgNP
OSE
SN
AgNP
OSE
SN
AgNP
OSE
SN
AgNP
100
S
S
S
S
S
S
S
S
S
S
S
S
50
R
S
S
R
S
S
S
S
S
R
S
S
25
R
S
S
R
S
S
R
S
S
R
S
S
12.5
R
S
S
R
S
S
R
R
S
R
S
S
6.25
R
S
S
R
S
S
R
R
S
R
S
S
3.125
R
R
S
R
S
S
R
R
S
R
S
S
1.5
R
R
S
R
R
S
R
R
R
R
S
S
0.8
R
R
S
R
R
S
R
R
R
R
S
S
0.4
R
R
S
R
R
R
R
R
R
R
R
S
0.2
R
R
R
R
R
R
R
R
R
R
R
R

OSE: ocimum sanctum extract; SN: silver nitrate; AgNP: silver nanoparticles with ocimum extract; R: Resistant; S: Sensitive; Fn: Fusobacterium nucleatum; Pg: Porphyromonas gingivalis; Aa: Aggregatibacter actinomycetemcomitans; Pi: Prevotella intermedia
Table 1: MIC of test solutions

Serial
dilutions (μg/ml)
Fn (CFU/ml)
Pg (CFU/ml)
Aa (CFU/ml)
Pi (CFU/ml)
OSE
SN
AgNP
OSE
SN
AgNP
OSE
SN
AgNP
OSE
SN
AgNP
100
32
NG
28
48
8
34
NG
52
NG
NG
54
48
50
49
NG
30
54
20
39
34
60
NG
60
58
59
25
52
NG
39
76
27
43
42
62
NG
78
62
70
12.5
58
NG
54
79
30
49
48
70
NG
84
99
74
6.25
83
54
68
89
32
54
50
78
NG
96
102
98
3.125
103
62
74
90
76
60
68
88
NG
128
124
113
1.5
136
78
83
96
89
69
74
94
13
154
126
126
0.8
142
80
90
103
96
72
98
93
18
186
130
134
0.4
182
93
103
105
104
89
113
102
20
206
132
148
0.2
220
102
136
136
140
102
136
132
25
213
198
152

OSE : ocimum sanctum extract; SN: silver nitrate; AgNP: silver nanoparticles with ocimum extract; CFU/ml : Colony forming units per milli litre; Fn: Fusobacterium nucleatum; Pg: Porphyromonas gingivalis; Aa: Aggregatibacter actinomycetemcomitans; Pi: Prevotella intermedia
Table 2: MBC of test solutions